Enzymatic detection of Neisseria gonorrhoeae.
نویسندگان
چکیده
منابع مشابه
Enzymatic detection of Neisseria gonorrhoeae.
In a study using a non-serological enzymatic approach for the detection of Neisseria gonorrhoeae in cervical and urethral swabs, the technique was shown to be technically feasible. The enzyme, 1, 2-propanediol oxidoreductase, was used as a presumptive diagnostic marker for N gonorrhoeae. Enzymatic activity was measured with a fluorometer. Two assay procedures were performed: (a) enzyme detectio...
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During 1989 and 1990 strains of Neisseria gonorrhoeae with reduced susceptibility to ciprofloxacin were isolated in laboratories across the United Kingdom. Treatment failures were associated with some of these infections. These strains were detected by quantitative susceptibility testing because the zone of inhibition around 5 micrograms ciprofloxacin discs shows little decrease in size even wi...
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Two fi-lactamase-producing strains of Neisseria gonorrhoeae were studied. The substrate profile, molecular weight, and isoelectric point of their ,8-lactamases were similar to those of the TEM-1 enzyme produced by many gram-negative bacilli. The gonococcal f8-lactamase was cell bound during exponential growth and was most likely located in the periplasm. Penicillin hydrolysis was efficient in i...
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An enzyme immunoassay (EIA; Gonozyme, Abbott Laboratories) for the detection of Neisseria gonorrhoeae antigens was assessed. Clinical isolates of N. gonorrhoeae or specimens obtained from male urethra or female cervix were tested by EIA. EIA results were compared with results of conventional culture identification for N. gonorrhoeae. Fifty clinical isolates of gonococci reacted positively with ...
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ژورنال
عنوان ژورنال: Sexually Transmitted Infections
سال: 1980
ISSN: 1368-4973
DOI: 10.1136/sti.56.5.304